Figure 2.

'Reward pool' genes characterize the transcriptional response to amphetamine-triggered CPP. (a) Diagram of differentially expressed genes from microarray experiments. Individual microarray experiments were combined to reveal a reward pool. A comparison of the differential expression from two experiments showed no bias in the direction of expression. Pool 1 shows the genes differentially expressed in 'wild type with amphetamine versus wild-type without amphetamine'. Pool 2 represents genes differentially expressed in 'mutant with amphetamine versus sibling with amphetamine'. Pool 3 represents genes differentially expressed in 'mutant without amphetamine versus non-mutant siblings without amphetamine'. The genes in pool 3 were subtracted from pool 2 in order to eliminate basal differences between mutants and siblings, not due to amphetamine administration. The intersection of the remaining genes in pool 2 and the genes in pool 1 forms the 'reward pool'. The genes in this pool are differentially expressed in both experiments - that is, they are involved in the wild-type response to amphetamine, as well as the non-response to amphetamine in the mutant. (b) Comparison of the direction of regulation (up or down) of transcripts from the reward pool for the experiments wt+/wt- and mut+/sib+. No bias towards a particular pattern can be observed.