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Comparative genome and phenotypic analysis of Clostridium difficile 027 strains provides insight into the evolution of a hypervirulent bacterium

Richard A Stabler1, Miao He2, Lisa Dawson1, Melissa Martin1, Esmeralda Valiente1, Craig Corton2, Trevor D Lawley2, Mohammed Sebaihia2, Michael A Quail2, Graham Rose2, Dale N Gerding3, Maryse Gibert4, Michel R Popoff4, Julian Parkhill2, Gordon Dougan2 and Brendan W Wren1*

Author Affiliations

1 London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK

2 Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK

3 Hines VA Hospital, Hines, IL 60141, USA

4 Institut Pasteur, rue du Dr Roux, 75724, Paris, France

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Genome Biology 2009, 10:R102  doi:10.1186/gb-2009-10-9-r102

Published: 25 September 2009

Additional files

Additional data file 1:

CDS specific to PCR-ribotype 027 isolates.

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Additional data file 2:

Fourteen CDSs have been disrupted by an insertion in both 027 strains but are intact in 630, and, conversely, 12 CDSs are intact in both 027 strains but have been disrupted in 630.

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Additional data file 3:

R20291-specific gene primers used in this study.

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Additional data file 4:

Toxin B was quantified by gel densitometry. 1 = VPI10463, 2 = CD196, 3 = 630.

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