Genome Biology

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Open Access Research

Genome-wide deletion mutant analysis reveals genes required for respiratory growth, mitochondrial genome maintenance and mitochondrial protein synthesis in Saccharomyces cerevisiae

Sandra Merz1 and Benedikt Westermann1,2*

Author Affiliations

1 Institut für Zellbiologie, Universität Bayreuth, Universitätsstraße 30, 95440 Bayreuth, Germany

2 Bayreuther Zentrum für Molekulare Biowissenschaften (BZMB), Universität Bayreuth, Universitätsstraße 30, 95440 Bayreuth, Germany

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Genome Biology 2009, 10:R95 doi:10.1186/gb-2009-10-9-r95

Published: 14 September 2009

Additional files

Additional data file 1:

pet genes isolated from the MATα deletion library.

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Additional data file 2:

pet genes unique to this study.

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Additional data file 3:

pet genes grouped according to their occurrence in pet screens and localization and function of the encoded gene products.

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Additional data file 4:

pet genes producing growth defects only on specific carbon sources.

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Additional data file 5:

Mutants belonging to four classes of pet genes.

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Additional data file 6:

Quantification of loss of mtDNA in class IV pet mutants.

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Additional data file 7:

pet genes dispensable for respiration.

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Additional data file 8:

Genes required for respiratory activity in class I and III pet mutants that show a wild-type pattern of mitochondrial translation products.

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Additional data file 9:

Genes possibly affecting mitochondrial function in combination with acquired defects.

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Additional data file 10:

Primers used to confirm the identity of yeast deletion mutants.

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