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Rapid chromosome territory relocation by nuclear motor activity in response to serum removal in primary human fibroblasts

Ishita S Mehta1, Manelle Amira12, Amanda J Harvey2 and Joanna M Bridger1*

Author Affiliations

1 Centre for Cell and Chromosome Biology, Division of Biosciences, School of Health Sciences and Social Care, Brunel University, Kingston Lane, Uxbridge, UB8 3PH, UK

2 Brunel Institute for Cancer Genetics and Pharmacogenomics, Division of Biosciences, School of Health Sciences and Social Care, Brunel University, Kingston Lane, Uxbridge, UB8 3PH, UK

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Genome Biology 2010, 11:R5  doi:10.1186/gb-2010-11-1-r5

Published: 13 January 2010

Additional files

Additional data file 1:

The chromosome position of chromosomes 10, 13, 18, and X 30 minutes after serum removal from a proliferating culture of human dermal fibroblasts in a 2D study (1A-D), and the 3D analysis of the nuclear position of chromosomes 10 and X after 15 minutes after serum removal from a proliferating culture (1E).

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Additional data file 2:

Treating cells with 0.1% DMSO, in which the drugs are dissolved, does not interfere with the chromosome-repositioning response.

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Additional data file 3:

3D analyses of chromosome position for chromosomes 10 and X after treatment with GTPase inhibitor AG10 and serum removal (3A), after treatment with phalloidin oleate and serum removal (3B) and after treatment with BDM and serum removal (3C).

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Additional data file 4:

The DAPI distribution with each shell of the 2D erosion analysis script for each experiment performed, revealing that the DNA content did not alter after any of the treatments (4).

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Additional data file 5:

A table describing the inhibitors and drugs used in this study.

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Open Data