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Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data

Manuela Marullo1, Chiara Zuccato1, Caterina Mariotti2, Nayana Lahiri3, Sarah J Tabrizi3, Stefano Di Donato2 and Elena Cattaneo1*

Author Affiliations

1 Department of Pharmacological Sciences and Center for Stem Cell Research, University of Milan, 9 Via Balzaretti, Milan, 20133, Italy

2 Division of Biochemistry and Genetics, National Neurological Institute-IRCCS "Carlo Besta", 11 Via Celoria, Milan, 20133, Italy

3 Department of Neurodegenerative Disease, UCL Institute of Neurology University College London and National Hospital for Neurology and Neurosurgery, Queen Square, London, WC1N 3BG, UK

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Genome Biology 2010, 11:R9  doi:10.1186/gb-2010-11-1-r9

Published: 28 January 2010

Additional files

Additional file 1:

Protocol for EAR amplification in the human transcriptome.

Format: PDF Size: 33KB Download file

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Open Data

Additional file 2:

A selection of RNA from peripheral blood used in this study.

Format: PDF Size: 176KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data