Table 1

Primers sequences for GeNorm

Symbol

Name

NCBI accession number

Localization

Span intro

Sequences


ACTB

Beta actin

NC_001101

Chromosome 7

No

Fw:5'-AGTGTGACGTGGACATCCGCA-3'

Rev:5'-GCCAGGGCAGTGATCTCCTTCT-3'

HPRT1

Hypoxanthine purine synthesis phosphoribosyl-transferase 1

NC_000023.9

Chromosome x

Yes

Fw:5'-ATGACCAGTCAACAGGGGACAT-3'

Rev: 5'-CAACACTTCGTGGGGTCCTTTTCA-3'

YWHAZ

Tyrosine 3-monooxygenase/S tryptophan 5-monooxygenase activation protein, zeta polypeptide

NC_000008.9

Chromosome 8

Yes

Fw: 5'-CGTTACTTGGCTGAGGTTGCC-3'

Rev: 5'-GTATGCTTGTTGTGACTGATCGAC-3'

GNB2L1

Guanine nucleotide binding protein, beta polypeptide 2-like 1

NC_000005.8

Chromosome 5

Yes

Fw: 5'-GAGTGTGGCCTTCTCCTCTG-3'

Rev: 5'-GCTTGCAGTTAGCCAGGTTCC-3'

B2M

Beta-2-microglobulin

NC_000015.8

Chromosome 15

No

Fw: 5'-GGAGAGAGAATTGAAAAAGTGGAGC-3'

Rev: 5'-GGCTGTGACAAAGTCACATGGTT-3'

GAPDH

Glyceraldehyde-3-oxidoreductase phosphate dehydrogenase

NC_000012.10

Chromosome 12

No

Fw: 5'-AGCTGAACGGGAAGCTCACT-3'

Rev: 5'-AGGTCCACCACTGACACGTTG-3'


Marullo et al. Genome Biology 2010 11:R9   doi:10.1186/gb-2010-11-1-r9

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