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Immunostaining of modified histones defines high-level features of the human metaphase epigenome

Edith Terrenoire1,2†, Fiona McRonald1,3†, John A Halsall1, Paula Page2, Robert S Illingworth4,5, A Malcolm R Taylor6, Val Davison2, Laura P O'Neill1 and Bryan M Turner1*

1 Chromatin and Gene Expression Group, Institute of Biomedical Research, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK

2 West Midlands Regional Genetics Laboratory, Birmingham Women's NHS Foundation Trust, Metchley Park Road, Edgbaston, Birmingham B15 2TG, UK

3 Current address: School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK

4 The Wellcome Trust Centre for Cell Biology, University of Edinburgh, The King's Buildings, Edinburgh EH9 3JR, UK

5 Current address: MRC Human Genetics Unit, Crewe Road, Edinburgh EH4 2XU, UK

6 School of Cancer Sciences, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK

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Genome Biology 2010, 11:R110 doi:10.1186/gb-2010-11-11-r110

Published: 15 November 2010

Additional files

Additional file 1:

Figure showing relative lengths of human chromosomes with and without fixation in methanol acetic acid.

Format: TIFF Size: 411KB Download file

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Additional file2:

Figure showing centromeric indices of human chromosomes with and without fixation in methanol acetic acid.

Format: TIFF Size: 459KB Download file

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Additional file 3:

Figure showing alignment of individual metaphase chromosomes immunostained for H3K4me3 from five different chromosome spreads.

Format: TIFF Size: 8.7MB Download file

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Additional file 4:

Figure showing alignment of individual metaphase chromosomes immunostained for H3K27ac from six different chromosome spreads.

Format: TIFF Size: 7.6MB Download file

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Additional file 5:

Figure showing alignment of individual metaphase chromosomes immunostained for H3K9ac from two different chromosome spreads.

Format: TIFF Size: 5.2MB Download file

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Additional file 6:

Figure showing alignment of individual metaphase chromosomes immunostained for H3K27me3 from six different chromosome spreads.

Format: TIFF Size: 7.5MB Download file

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Additional file 7:

Figure showing the correspondence between gene density, CpG island density and H3K27me3 levels across human metaphase chromosomes.

Format: TIFF Size: 4.4MB Download file

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Additional file 8:

Table showing antibodies used for labeling and their origins.

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Additional file 9:

Immunostained karyotype showing the distribution of H3K4me3 across human fibroblast chromosomes.

Format: TIFF Size: 1.5MB Download file

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Additional file 10:

Table showing correlations between H3K4me3 levels across metaphase chromosome 1, gene-, CGI- and repeat-frequencies and various properties of the interphase epigenome.

Format: DOCX Size: 13KB Download file

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