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Caenorhabditis elegans chromosome arms are anchored to the nuclear membrane via discontinuous association with LEM-2

Kohta Ikegami1, Thea A Egelhofer2, Susan Strome2 and Jason D Lieb1*

Author Affiliations

1 Department of Biology, Carolina Center for Genome Sciences and Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, 407 Fordham Hall, Chapel Hill, North Carolina 27599, USA

2 Department of MCD Biology, University of California Santa Cruz, 1156 High Street, Santa Cruz, California 95064, USA

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Genome Biology 2010, 11:R120  doi:10.1186/gb-2010-11-12-r120

Published: 23 December 2010

Additional files

Additional file 1:

Figures S1 to S8. Figure S1: antibody characterization. Figure S2: LEM-2 domains on individual chromosomes. Figure S3: distribution of LEM-2 subdomains along chromosomes. Figure S4: coverage of different types of repetitive sequences in LEM-2 subdomains and gaps. Figure S5: LEM-2 association status of genes for which subnuclear positions were previously determined by fluorescent in situ hybridization (FISH). Figure S6: validation of a strain with a fusion chromosome. Figure S7: coverage of helitrons and satellite repeats in LEM-2 subdomains along chromosomes. Figure S8: LEM-2 subdomain calling.

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Additional file 2:

Tables S1 to S7. Tables are in individual tabs in Additional file 2 (Microsoft Excel file). Table S1: LEM-2 subdomains. Table S2: gaps between LEM-2 subdomains. Table S3: the number of genes in LEM-2 subdomains and gaps. Table S4: ChIP-chip, ChIP-seq and expression profiling performed in this study. Table S5: oligonucleotide sequences used in this study. Table S6: LEM-2 subdomain-gap boundaries analyzed in this study. Table S7: genome coordinates for chromosome arms and central regions.

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