Additional file 6.

NO3- presence enhances pSPL9:rSPL9 phenotypes. Wild type and pSPL9:rSPL9 have been plated on the same background media as used for kinetic experiments (see Materials and methods) and complemented with either 0.5 mM (NH4)2-succinate or 1 mM KNO3. Eight days after germination, different root traits were scored (n = 13 to 24 plants). ANOVA was used to evaluate the genotype effect (Geno: wild type versus pSPL9:rSPL9); the treatment effect (Treat: nitrate versus ammonium) and the interaction of these two factors (G × T). P-values are provided above each plot corresponding to different root parameters. ***P < 0.001; **P < 0.01; *P < 0.05. NO3- presence significantly enhances transgene effect for lateral root number, total lateral root length, lateral root density, and lateral root length density. The average length of lateral roots is not significantly controlled by the pSPL9:rSPL9 transgene.

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Krouk et al. Genome Biology 2010 11:R123   doi:10.1186/gb-2010-11-12-r123