Method
A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454
-
* Corresponding author: Robert Nicol nicol@broadinstitute.org
1 Genome Sequencing Platform, Broad Institute of MIT and Harvard, 320 Charles St., Cambridge, MA 02141, USA
2 Current address: Network Control Engineering, Akamai Technologies Inc., 8 Cambridge Center, Cambridge, MA 02142, USA
3 Current address: Engineering, Google Inc., 5 Cambridge Center, Cambridge, MA 02142, USA
4 Genome Sequencing and Analysis Program, Broad Institute of MIT & Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA
5 Current address: Genomic Technologies, Joint Genome Institute, Walnut Creek, CA 94598, USA
Genome Biology 2010, 11:R15 doi:10.1186/gb-2010-11-2-r15
Published: 5 February 2010Additional files
Additional file 1:
A Word document containing details and methods referred to but not described in the text.
Format: DOC Size: 158KB Download file
This file can be viewed with: Microsoft Word Viewer
Additional file 2:
A figure containing a process map for plate-based fragment library construction with details of automation used for each step.
Format: PPT Size: 196KB Download file
This file can be viewed with: Microsoft PowerPoint Viewer
Additional file 3:
A figure containing a process map for plate-based 3-kb jumping library construction with details of automation used for each step.
Format: PPT Size: 260KB Download file
This file can be viewed with: Microsoft PowerPoint Viewer
Additional file 4:
A figure illustrating variation in library yield across the plate.
Format: PDF Size: 1.2MB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional file 5:
A figure illustrating the layout of 24 samples in a 96-well plate.
Format: PPTX Size: 660KB Download file