Additional file 2.

Cell cycle progression in nocodazole-treated versus untreated cells. A PDF figure file showing cell cycle progression in nocodazole-treated versus untreated cells. (a) Flow histograms plotting DNA content against cell number (left) and histograms indicating the ploidy distribution (2C versus 4C; right) during a 12-h time course of synchronized cells in the absence of nocodazole. At the later time points (t = 10 to 12), the level of synchrony decreased, indicated by the ploidy level equilibrium reached at these time points, probably resulting from cells entering the next cell cycle round, while other cells still have to pass through M phase. (b) Flow histograms plotting DNA content against cell number (left) and histograms indicating the ploidy distribution (2C versus 4C; right) during a 12-h time course of synchronized cells in the presence of nocodazole. At the later time points, an increasing enrichment of 4C cells can be observed because of a blockage of the cells at metaphase. Asterisk marks the apparently lower proportion of 2C cells after a 20-h dark treatment in the control series than in the nocodazole series, resulting from an acquisition artefact during flow cytometry, indicated by the increased peak broadness in the respective flow histogram.

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Huysman et al. Genome Biology 2010 11:R17   doi:10.1186/gb-2010-11-2-r17