Figure 7.

TFIIB and NC2 binding to TATA (+/-) promoters in nuclear extracts. PICs were formed on immobilized HIV/AdML promoter templates containing a wild-type (wt) or mutant (mt) TATA box using Jurkat nuclear extract under basal conditions (-VP16) or in the presence of the activator Gal4-VP16 (+VP16). After washing, the reactions were analyzed by immunoblotting with specific antibodies against TFIIB or NC2. Blots were scanned and quantified using ImageJ [41]. Bars and error bars represent mean and standard deviation of three independent reactions. TFIIB and NC2 template association is expressed as percentage of relative binding, with the reaction showing maximum binding set to 100%.