Polymorphic HpaII sites identified by HELP-tagging. Examples of HpaII sites (a) annotated in the reference genome sequence but not represented by MspI reads or (b) not annotated in the reference human genome and represented by at least four MspI reads are shown. In each case there is a SNP defined by dbSNP that indicates the C:T to G:A transversion that eliminates or restores the CCGG HpaII site.
Suzuki et al. Genome Biology 2010 11:R36 doi:10.1186/gb-2010-11-4-r36