Improving draft assemblies by iterative mapping and assembly of short reads to eliminate gaps
Citation and License
Genome Biology 2010, 11:R41 doi:10.1186/gb-2010-11-4-r41Published: 13 April 2010
Advances in sequencing technology allow genomes to be sequenced at vastly decreased costs. However, the assembled data frequently are highly fragmented with many gaps. We present a practical approach that uses Illumina sequences to improve draft genome assemblies by aligning sequences against contig ends and performing local assemblies to produce gap-spanning contigs. The continuity of a draft genome can thus be substantially improved, often without the need to generate new data.