Summary of the ChIP-seq results using bam testis. (a) The four groups of genes were classified according to their RPKM value based on the RNA-seq results . The numbers in brackets denote genes used for H3K36me3 (K36) analysis. *See Materials and methods for gene selection criteria. Antibodies used for ChIP-seq were: (b) anti-RNA Pol II (Pol II); (c) anti-H3K4me3 (K4); (d) anti-H3K36me3 (K36); and (e) anti-H3K27me3 (K27). Enrichment of each histone modification and RNA Pol II is plotted over a -3-kb to +3-kb region with respect (w.r.t.) to genes' TSSs, except for K36, for which a -5-kb to +5-kb region is used.
Gan et al. Genome Biology 2010 11:R42 doi:10.1186/gb-2010-11-4-r42