Additional file 4.

Validation of bivalent genes using ChIP followed by real-time PCR analyses on five gene loci. (a) Rpl7A gene is used as a positive control for the active H3K4me3 enrichment and don juan (dj) is used as a positive control for the repressive H3K27me3 mark. All five bivalent genes have H3K4me3 and H3K27me3 at levels between the two controls. The y-axis denotes the ChIP-ed DNA/input DNA percentage. Primer pairs used for PCR validation are shown as black boxes in each panel of (b-f). Primer sequence information is in Materials and methods. (b-f) UCSC genome browser screenshots showing H3K4me3 and H3K27me3 enrichment at each of the following genes' loci: (b) bgcn; (c) Neurochondrin; (d) CG14834; (e) NPC2; and (f) retn.

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Gan et al. Genome Biology 2010 11:R42   doi:10.1186/gb-2010-11-4-r42