Figure 6.

The effects of Tsc22d3 and Sgk1 knockdown on dendritic spine morphology in cultured primary neurons. Representative micrographs and three-dimensional Imaris reconstructions of dendritic segments of hippocampal and cortical neurons are presented. The neurons were transfected with pSUPER (control) or GILZsh mix or SGK1sh mix in pSUPER on day in vitro 14 for 3 days. GFP was used to highlight transfected cell morphology.