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Open Access Research

Short RNA half-lives in the slow-growing marine cyanobacterium Prochlorococcus

Claudia Steglich12, Debbie Lindell13, Matthias Futschik45, Trent Rector67, Robert Steen6 and Sallie W Chisholm1*

Author Affiliations

1 Massachusetts Institute of Technology, Department of Civil and Environmental Engineering, Cambridge, MA 02139, USA

2 University of Freiburg, Faculty of Biology, D-79104 Freiburg, Germany

3 Technion - Israel Institute of Technology, Faculty of Biology, Haifa 32000, Israel

4 University of Algarve, Institute for Biotechnology and Bioengineering, Centre for Molecular and Structural Biomedicine, 8005-139 Faro, Portugal

5 Humboldt University, Institute for Theoretical Biology, Charité, 10115 Berlin, Germany

6 Harvard Medical School, Department of Genetics, Biopolymers Facility, Boston, MA 02115, USA

7 PerkinElmer Life and Analytical Sciences, Waltham, MA 02451, USA

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Genome Biology 2010, 11:R54  doi:10.1186/gb-2010-11-5-r54

Published: 19 May 2010

Abstract

Background

RNA turnover plays an important role in the gene regulation of microorganisms and influences their speed of acclimation to environmental changes. We investigated whole-genome RNA stability of Prochlorococcus, a relatively slow-growing marine cyanobacterium doubling approximately once a day, which is extremely abundant in the oceans.

Results

Using a combination of microarrays, quantitative RT-PCR and a new fitting method for determining RNA decay rates, we found a median half-life of 2.4 minutes and a median decay rate of 2.6 minutes for expressed genes - twofold faster than that reported for any organism. The shortest transcript half-life (33 seconds) was for a gene of unknown function, while some of the longest (approximately 18 minutes) were for genes with high transcript levels. Genes organized in operons displayed intriguing mRNA decay patterns, such as increased stability, and delayed onset of decay with greater distance from the transcriptional start site. The same phenomenon was observed on a single probe resolution for genes greater than 2 kb.

Conclusions

We hypothesize that the fast turnover relative to the slow generation time in Prochlorococcus may enable a swift response to environmental changes through rapid recycling of nucleotides, which could be advantageous in nutrient poor oceans. Our growing understanding of RNA half-lives will help us interpret the growing bank of metatranscriptomic studies of wild populations of Prochlorococcus. The surprisingly complex decay patterns of large transcripts reported here, and the method developed to describe them, will open new avenues for the investigation and understanding of RNA decay for all organisms.