Distribution of miRNA binding sites in sense-antisense RNA transcripts. Predicted miRNA binding sites were counted in 391 human sense-antisense pairs. The total number of predicted miRNAs per 100 nucleotides of overlapping and non-overlapping regions of each sense-antisense pair is depicted. The miRNA binding sites within pairing and non-pairing regions have a median value of 4.78 and 6.49 miRNAs per 100 nucleotides, respectively. On average (mean), each 100 nucleotides of overlapping region have 5.21 miRNA binding sites, while each 100 nucleotides of non-overlapping region have 7.07 miRNA binding sites. The difference seen in miRNA numbers within the sense-antisense pairing regions and non-pairing regions is statistically significant (***P-value < 0.0001, Wilcoxon two-sided test).
Faghihi et al. Genome Biology 2010 11:R56 doi:10.1186/gb-2010-11-5-r56