Sensitivity and specificity as a function of the amount of sequence data generated. Shown for all exons, core exons, and exons that are well expressed in PBMCs, designated as an expression level of at least 4% of the most highly expressed transcript in PBMCs. There were approximately 35 million sequence reads in each lane.
Cirulli et al. Genome Biology 2010 11:R57 doi:10.1186/gb-2010-11-5-r57