Method
Global fitness profiling of fission yeast deletion strains by barcode sequencing
- † Equal contributors
National Institute of Biological Sciences, 7 Science Park Road, Zhongguancun Life Science Park, Beijing, 102206, PR China
Genome Biology 2010, 11:R60 doi:10.1186/gb-2010-11-6-r60
Published: 10 June 2010Abstract
A genome-wide deletion library is a powerful tool for probing gene functions and one has recently become available for the fission yeast Schizosaccharomyces pombe. Here we use deep sequencing to accurately characterize the barcode sequences in the deletion library, thus enabling the quantitative measurement of the fitness of fission yeast deletion strains by barcode sequencing.
