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Open Access Method

Global fitness profiling of fission yeast deletion strains by barcode sequencing

Tian Xu Han, Xing-Ya Xu, Mei-Jun Zhang, Xu Peng and Li-Lin Du*

Author Affiliations

National Institute of Biological Sciences, 7 Science Park Road, Zhongguancun Life Science Park, Beijing, 102206, PR China

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Genome Biology 2010, 11:R60  doi:10.1186/gb-2010-11-6-r60

Published: 10 June 2010

Abstract

A genome-wide deletion library is a powerful tool for probing gene functions and one has recently become available for the fission yeast Schizosaccharomyces pombe. Here we use deep sequencing to accurately characterize the barcode sequences in the deletion library, thus enabling the quantitative measurement of the fitness of fission yeast deletion strains by barcode sequencing.