Additional file 8.
The linearity and dynamic range of barcode sequencing assessed using spike-in controls. A rad32 deletion strain and a rad26 deletion strain from the Bioneer version 1.0 upgrade package (M-1030H-U) were spiked into 24 version 1.0 pooled samples that had been grown in minimal or rich medium for different generations. The ratios between the cell number of each spike-in strain and the total cell number of the version 1.0 pooled strains were 1/200, 1/1,000, 1/2,500, 1/5,000, 1/10,000, and 1/20,000. The read numbers were normalized by total matched reads of the version 1.0 strains. (a) The normalized read numbers were plotted against the spike-in ratios. (b) The observed log fold changes between different spike-in samples were plotted against expected log fold changes.
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Han et al. Genome Biology 2010 11:R60 doi:10.1186/gb-2010-11-6-r60