Schematic representation of the experimental strategy used. A set of 32 genes, 29 murine orthologs of HSA21 genes and 3 human coding sequences, were cloned into the pPthC vector  and nucleofected along with a pCAGGS-Cre recombinase vector  into EBRTcH3 (EB3) cells. Puromycin-resistant clones were isolated and grown in medium deprived of tetracycline for varying periods of time to perform a time course of induction. The inducibility of selected clones was evaluated by q-PCR. Global transcriptome and proteome analysis was performed by hybridization onto an Affymetrix gene chip and by large-gel two-dimensional gel electrophoresis (2DGE), respectively, to delineate the consequences of gene dosage imbalance on a single gene basis. WB, western blot.
De Cegli et al. Genome Biology 2010 11:R64 doi:10.1186/gb-2010-11-6-r64