Table 2

Correlation between differential protein expression by 2DGE (protein ratio) and differential gene expression by microarray (mRNA ratio)

Spot ID*

Gene

Protein name

Protein ratio 48 h/0 h*

mRNA ratio 24 h/0 h


3214

Uchl1

Ubiquitin carboxy-terminal hydrolase L1

0.58

0.63

662

Dppa4

Developmental pluripotency associated 4 isoform 1

0.65

0.75

3549

Igf2bp2

Insulin-like growth factor 2 mRNA-binding protein 2 (IGF-II mRNA-binding protein 2) (IMP-2)

0.65

1.39

2836

Sipa1l1

Signal-induced proliferation-associated 1-like protein 1

0.74

1.54

425

Lap3

Cytosol aminopeptidase (Leucine aminopeptidase) (LAP) (Leucyl aminopeptidase) (Leucine aminopeptidase 3) (Proline aminopeptidase)(Prolyl aminopeptidase)

0.76

0.72

2512

Hspd1

60 kDa heat shock protein, mitochondrial precursor (Hsp60) (60 kDa chaperonin) (CPN60) (Heat shock protein 60) (HSP-60) (Mitochondrial matrix protein P1) (HSP-65)

0.78

0.75

3410

Eif1a

Eukaryotic translation initiation factor 1A, Y-linked

0.8

0.76

403

Pkm2

Pyruvate kinase isozyme M2

1.26

3.05

935

Bdh1

D-beta-hydroxybutyrate dehydrogenase, mitochondrial precursor

1.31

1.53

645

Serpinh1

Serine (or cysteine) proteinase inhibitor, clade H, member 1

1.44

1.36

2920

Ldhb

Lactate dehydrogenase 2, B chain

1.46

0.64

3562

Cotl1

Coactosin-like 1

1.62

1.39

3653

S100a11

S100 calcium binding protein A11 (calizzarin)

1.89

1.18

3144

Sept1

Septin 1

2.28

0.71

1134

Gsto1

Glutathione S-transferase omega 1

2.77

3.73

3588

Fabp3

Fatty acid binding protein 3, muscle and heart

3.05

2.2

3078

Apoe

Apolipoprotein E

3.28

0.8


Only two gene/protein pairs, apoE and Sept1, showed bifurcated regulation in protein and microarray assays.

De Cegli et al. Genome Biology 2010 11:R64   doi:10.1186/gb-2010-11-6-r64

Open Data