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Long noncoding RNA genes: conservation of sequence and brain expression among diverse amniotes

Rebecca A Chodroff12, Leo Goodstadt3, Tamara M Sirey1, Peter L Oliver3, Kay E Davies13, Eric D Green2, Zoltán Molnár1* and Chris P Ponting13*

Author Affiliations

1 Department of Physiology, Anatomy, and Genetics, Le Gros Clark Building South Parks Road, University of Oxford, Oxford OX1 3QX, UK

2 Genome Technology Branch, National Human Genome Research Institute, National Institutes of Health, 50 South Drive, Building 50, Room 5222, Bethesda, MD 20892, USA

3 MRC Functional Genomics Unit, Le Gros Clark Building, South Parks Road, University of Oxford, Oxford OX1 3QX, UK

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Genome Biology 2010, 11:R72  doi:10.1186/gb-2010-11-7-r72

Published: 12 July 2010

Additional files

Additional file 1:

Figure S1: splice-site and poly(A)-signal conservation among AK043754, AK082072, and AK082467 orthologs. Figure S2: sense probe controls for in situ hybridization.

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Open Data

Additional file 2:

Table S1: genome coordinates used in multi-species sequence alignments. Table S2: PCR primers used for amplification of in situ hybridization probes and 3' and 5' lncRNA ortholog RACE.

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Open Data