Figure 5.

Analysis of PolII occupancy in the rpb1-1 mutant. (a) Examples of time course data from wild type (wt; left panels) or rpb1-1 yeast (right panels) during heat shock time courses. Chromosome coordinates are indicated between the two sets of panels. Note the decrease in dynamic range in the right panels in the last two columns, manifest as decreased color saturation in the rightmost two columns. (b, c) Histograms of microarray probe values for wild-type (b) or rpb1-1 (c) cells at varying times. Narrowing of the histogram in (c) indicates loss of PolII enrichment after approximately 1 hour of treatment with the restrictive temperature.