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This article is part of the supplement: Beyond the Genome: The true gene count, human evolution and disease genomics

Invited speaker presentation

The developmental transcriptome of Drosophila melanogaster

Brenton Graveley

  • Correspondence: Brenton Graveley

Author Affiliations

Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030-3301, USA

Genome Biology 2010, 11(Suppl 1):I11  doi:10.1186/gb-2010-11-s1-i11

The electronic version of this article is the complete one and can be found online at: http://genomebiology.com/2010/11/S1/I11


Published:11 October 2010

© 2010 Graveley; licensee BioMed Central Ltd.

Invited speaker presentation

Drosophila melanogaster is one of the well-studied metazoan organisms; nonetheless its genome still contains unannotated genes, exons and RNA editing sites. Furthermore, our understanding of how the regulation of transcription, splicing and RNA editing directs development of this complex organism remains limited. We have used RNA-seq, tiling microarrays and cDNA sequencing to explore the transcriptome in 30 distinct stages throughout development. We have identified 87,352 new features, including thousands of genes, coding and non-coding transcripts, exons, splicing and editing events. We have inferred protein isoforms that previously eluded discovery with established experimental, prediction and conservation-based approaches. Together, these data substantially expand the number of known transcribed elements in the Drosophila genome and provide a high-resolution view of transcriptome dynamics throughout the development of a metazoan.