Table 2

Automated solution hybrid selection performance

Performance factor

3 μg input average (n = 1,117 exomes)


Median target coverage

131.0×

Percentage bases > 2×

96.0%

Percentage bases > 10×

91.9%

Percentage bases > 20×

87.6%

Percentage selected bases (on target)

83.7%

Percentage duplicated reads

4.4%

Fold 80 penaltya

3.17

Estimated library size of captured fragments

278 million


See Additional file 12 for metric definitions. aFold 80 penalty is a measure of the non-uniformity of sequence coverage, defined as the amount of additional coverage (in fold coverage of the genome) required so that 80% of the target bases will be covered at the current mean coverage (see Additional file 12 for details).

Fisher et al. Genome Biology 2011 12:R1   doi:10.1186/gb-2011-12-1-r1

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