Table 2 |
|
|
Automated solution hybrid selection performance |
|
|
Performance factor |
3 μg input average (n = 1,117 exomes) |
|
|
|
|
Median target coverage |
131.0× |
|
Percentage bases > 2× |
96.0% |
|
Percentage bases > 10× |
91.9% |
|
Percentage bases > 20× |
87.6% |
|
Percentage selected bases (on target) |
83.7% |
|
Percentage duplicated reads |
4.4% |
|
Fold 80 penaltya |
3.17 |
|
Estimated library size of captured fragments |
278 million |
|
|
|
|
See Additional file 12 for metric definitions. aFold 80 penalty is a measure of the non-uniformity of sequence coverage, defined as the amount of additional coverage (in fold coverage of the genome) required so that 80% of the target bases will be covered at the current mean coverage (see Additional file 12 for details). |
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|
Fisher et al. Genome Biology 2011 12:R1 doi:10.1186/gb-2011-12-1-r1 |
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