Table 3 |
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|
Performance comparison of manual versus automated solution hybrid selection |
|||
|
Factor |
Column based |
Automated (with-bead SPRI) |
Automated (with-bead SPRI) low input |
|
|
|||
|
Input DNA |
3 μg |
3 μg |
0.1 μg |
|
Samples/FTE/week |
6-12 |
384 |
384 |
|
Number of sample transfer steps |
10 |
4 |
4 |
|
Output DNA prior to PCR |
720 ng |
1,330 ng |
Below limit of detection |
|
Number of pond PCR cycles |
12-16 |
6 |
6 |
|
Percentage duplicated reads |
19.8 |
2.2 |
10 |
|
Percentage selected bases |
84.7 |
88.6 |
83.76 |
|
Estimated library size |
43 million |
516 million |
223 million |
|
|
|||
|
FTE, Full time employee; SHS, solution hybrid selection; SPRI, solid-phase reversible immobilization. |
|||
|
Fisher et al. Genome Biology 2011 12:R1 doi:10.1186/gb-2011-12-1-r1 |
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