Table 3

Performance comparison of manual versus automated solution hybrid selection

Factor

Column based

Automated (with-bead SPRI)

Automated (with-bead SPRI) low input


Input DNA

3 μg

3 μg

0.1 μg

Samples/FTE/week

6-12

384

384

Number of sample transfer steps

10

4

4

Output DNA prior to PCR

720 ng

1,330 ng

Below limit of detection

Number of pond PCR cycles

12-16

6

6

Percentage duplicated reads

19.8

2.2

10

Percentage selected bases

84.7

88.6

83.76

Estimated library size

43 million

516 million

223 million


FTE, Full time employee; SHS, solution hybrid selection; SPRI, solid-phase reversible immobilization.

Fisher et al. Genome Biology 2011 12:R1   doi:10.1186/gb-2011-12-1-r1

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