Figure 1.

Hyphae and microconidia of A. benhamiae on human hair and human keratinocytes. (a) Fluorescence microscopic picture (laser scanning microscope LSM 5 LIVE, Zeiss, Jena) of hyphae and microconidia stained with fluorescent brightener 28 (Sigma, USA). Scale bar: 5 μm. (b) Colonization of human hair. Cyan, fluorescence brightener 28-stained fungal hyphae; orange, hair autofluorescence. Scale bar: 20 μm. (c) Attachment of microconidia to human keratinocytes. Cyan, fluorescence brightener 28-stained fungal hyphae, red, wheat-germ agglutinin stained keratinocytes. Scale bar: 5 μm. (d) Human keratinocytes with germinating A. benhamiae microconidia. Scanning electron microscopy image. Scale bar: 10 μm. See Additional file 1 for supplementary information pertaining to this figure.