Quantitative PCR quantification of PCR products. Quantitative PCR (qPCR) assay designed to detect and quantify all amplifiable solexa molecules (using oligos p5/p7 and SybrGreen) or shRNA-specific PCR products (using Taqman, amplification primers p5/p7 and a dual-labeled probe). (a) shRNA PCR products quantified against a library of known concentration. (b) Standard curve constructed using a ten-fold dilution series covering 100, 10, 1 and 0.1 pM. (c) Agilent electrophoresis profile of reference library.
Sims et al. Genome Biology 2011 12:R104 doi:10.1186/gb-2011-12-10-r104