Additional file 1.

Engineered depletion of shRNAs. To establish the sensitivity of the screening system, we performed a series of engineered depletion experiments. We manually altered the representation of constructs in a 10,000 shRNA screening pool so that approximately 1,000 hairpins were depleted by 75%, approximately 1,000 depleted by 50% and approximately 1,000 depleted by 25%.

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Sims et al. Genome Biology 2011 12:R104   doi:10.1186/gb-2011-12-10-r104