Additional file 2.

Detection of hairpin depletion at reduced read counts. Reads were sampled at random from an engineered depletion experiment involving approximately 10 million reads to give datasets of either approximately 5 million or approximately 2.5 million reads in total. shRNA depletion was estimated from these new datasets to show that depletion of 50% could be observed in datasets containing approximately 2.5 million reads.

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Sims et al. Genome Biology 2011 12:R104   doi:10.1186/gb-2011-12-10-r104