IMR90 GRO-seq read distribution along the length of actively transcribed genes. As reported previously , a significant spike in both sense (orange) and anti-sense (blue) reads is observed near the transcription start site (position 0 - TSS). A smaller spike is evident at the annotated gene end (position 1 - Gene end). As expected, very little GRO-seq signal is observed in non-genic regions (positions less than -0.5 and greater than 1.5), and RNA polymerase-mediated transcription continues past the annotated gene end (positions between 1 and 1.5).
Melgar et al. Genome Biology 2011 12:R113 doi:10.1186/gb-2011-12-11-r113