Figure 4.

BEST signature at IMR90 DNase hypersensitive sites and H3K27ac peak regions in IMR90 cells. (a-c) Signal for BEST (accumulation of GRO-seq sense reads accompanied by anti-sense reads immediately upstream) is shown at IMR90 DHSs located within actively transcribed intragenic regions (a), non-transcribed intragenic regions (b), and intergenic regions (c). Relative sense/plus read density (y-axis) is the sense/plus read density at a particular proportional position divided by the average sense/plus read density in the entire DHS + flanking region. Proportional positions between 0 and 1 on the x-axis correspond to the DHS peak. Positions < 0 and > 1 correspond to flanking regions. IMR90 DHSs and H3K37ac peaks potentially associated with promoters or gene ends were discarded from the analysis. Non-DHS control regions (black) were randomly generated and follow the same size distribution as DHSs.