Nonsense mutation in spr1178 and growth defect in penicillin-resistant S. pneumoniae. (a) The growth kinetics of S. pneumoniae R6 wild-type, S. pneumoniae R6SmR, 2x2b1a-M2 and S. pneumoniae R6SmR, 2x2b1a, spr1178-M2 was followed by measuring the optical density of the cultures every hour for a 20-hour period. (b) The reversion of the spr1178 nonsense mutation to a wild-type sequence decreased the growth defect of the R6M1 mutant. Results are the average of at least three independent measurements.
Fani et al. Genome Biology 2011 12:R115 doi:10.1186/gb-2011-12-11-r115