Table 2

Mutations identified in R6M1 and R6M2 penicillin-resistant mutants

S. pneumoniae strain

M1

M2


Locus name

Putative identification

Nucleotide change

Amino acid change

Nucleotide change

Amino acid change

spr0032

DNA polymerase I

A107G

H36R

spr0041

Transposase (orf2)

GAA31AAC

E11N

GCTCG36TCTCA

K12N;D14N

T63A

SYN

GG204TA

D69Y

spr0113

Hypothetical protein

A4G

K2E

spr0121

Surface protein PspA precursor

A4G

N2D

T1374C

SYN

spr0160

DNA mismatch repair protein (Hex B)

G752A

S251N

spr0182

Hypothetical protein

G82insertion

frame shift

spr0284

Alpha-xylosidase

T1107C

SYN

spr0304

Penicillin-binding protein 2x

A1150G

R384G

A842C

Q281P

G1552T

V518L

C1106T

A369V

C1654G

Q552E

A1150G

R384G

C1276T

R426C

G1552A

V518I

spr0329

Penicillin-binding protein 1a

G1630A

G544R

G1233A

W411*

spr0376

Conserved hypothetical protein

T207C

SYN

spr0422

Hypothetical protein

C391T

Q131*

spr0475

Conserved hypothetical protein

G318C

L106F

spr0509

Phenylalanyl-tRNA synthetase beta chain

C105T

SYN

spr0598

GTP-binding protein (TypA/BipA) (tyrosine phosphorylated protein A)

C905T

P302L

spr0666

ABC transporter ATP-binding protein - cell division (FtsE)

C410T

P137L

spr0764

30S Ribosomal protein S1

G1173A

M391I

spr0776

D-Alanyl-D-alanine carboxypeptidase

C287T

A96V

spr0866

Dihydroorotate dehydrogenase

C575T

P192L

spr0878

Exoribonuclease R

G280T

G94W

spr0895

Conserved hypothetical protein

G134C

R45T

spr0917

Citrulline cluster-linked gene

C59T

A20V

spr0934

ABC transporter substrate-binding protein - iron transport

G486A

W162*

spr1041

Hypothetical protein

C648T

SYN

spr1043

Conserved hypothetical protein

C109T

P37S

C105T

SYN

spr1092

tRNA pseudouridine 5S synthase

C210T

SYN

spr1127

Ribonuclease III

A228G

SYN

C554T

T185I

spr1152

LicD

G692T

P231L

spr1166

Signal recognition particle (Fifty four homolog)

C139T

P47S

spr1178

Hypothetical protein

C82T

Q28*

C82T

Q28*

spr1186

N-Acetylneuraminate lyase subunit, truncation

T603C

SYN

G620A

G207D

GAC660AAT

T221I

AA689CG

E230A

T696A

SYN

spr1224

Conserved hypothetical protein

T564C

SYN

spr1240

Alanyl-tRNA synthetase

A139G

T47A

spr1254

ABC transporter ATP-binding protein-phosphate transport (PstB)

G499A

G167S

C614T

T205I

spr1260

Conserved hypothetical protein

T597C

SYN

spr1272

N-Acetylglucosamine-6-phosphate isomerase

G385A

G129R

spr1384

UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysine ligase

G996A

M332I

spr1423

Conserved hypothetical protein

A673G

M225V

spr1453

Major facilitator superfamily transporter

T91C

F31L

spr1465

Conserved hypothetical protein

G419A

G140E

spr1517

Penicillin-binding protein 2b

C1245A

D415E

C1184T

A395V

C1528A

G665D

G1303A

G435S

A1351G

T451A

spr1587

Conserved hypothetical protein

C568T

SYN

spr1703

ABC transporter ATP-binding protein - oligopeptide transport

C575A

P192Q

spr1706

ABC transporter membrane-spanning permease - oligopeptide transport

T399deletion

frameshift

spr1862

Competence protein

A51G

SYN

spr1886

Degenerate transposase

A314G

*105W

spr1888

DNA mismatch repair protein (HexA)

C2183T

T728I

C976T

Q326*

spr1991

Glycerol kinase

GG78TT

E27*


Asterisks and SYN indicate nonsense mutations and synonymous mutations, respectively.

Fani et al. Genome Biology 2011 12:R115   doi:10.1186/gb-2011-12-11-r115

Open Data