Table 2 |
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|
Mutations identified in R6M1 and R6M2 penicillin-resistant mutants |
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|
S. pneumoniae strain |
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|
M1 |
M2 |
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|
|
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|
Locus name |
Putative identification |
Nucleotide change |
Amino acid change |
Nucleotide change |
Amino acid change |
|
spr0032 |
DNA polymerase I |
A107G |
H36R |
||
|
spr0041 |
Transposase (orf2) |
GAA31AAC |
E11N |
||
|
GCTCG36TCTCA |
K12N;D14N |
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|
T63A |
SYN |
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|
GG204TA |
D69Y |
||||
|
spr0113 |
Hypothetical protein |
A4G |
K2E |
||
|
spr0121 |
Surface protein PspA precursor |
A4G |
N2D |
||
|
T1374C |
SYN |
||||
|
spr0160 |
DNA mismatch repair protein (Hex B) |
G752A |
S251N |
||
|
spr0182 |
Hypothetical protein |
G82insertion |
frame shift |
||
|
spr0284 |
Alpha-xylosidase |
T1107C |
SYN |
||
|
spr0304 |
Penicillin-binding protein 2x |
A1150G |
R384G |
A842C |
Q281P |
|
G1552T |
V518L |
C1106T |
A369V |
||
|
C1654G |
Q552E |
A1150G |
R384G |
||
|
C1276T |
R426C |
||||
|
G1552A |
V518I |
||||
|
spr0329 |
Penicillin-binding protein 1a |
G1630A |
G544R |
G1233A |
W411* |
|
spr0376 |
Conserved hypothetical protein |
T207C |
SYN |
||
|
spr0422 |
Hypothetical protein |
C391T |
Q131* |
||
|
spr0475 |
Conserved hypothetical protein |
G318C |
L106F |
||
|
spr0509 |
Phenylalanyl-tRNA synthetase beta chain |
C105T |
SYN |
||
|
spr0598 |
GTP-binding protein (TypA/BipA) (tyrosine phosphorylated protein A) |
C905T |
P302L |
||
|
spr0666 |
ABC transporter ATP-binding protein - cell division (FtsE) |
C410T |
P137L |
||
|
spr0764 |
30S Ribosomal protein S1 |
G1173A |
M391I |
||
|
spr0776 |
D-Alanyl-D-alanine carboxypeptidase |
C287T |
A96V |
||
|
spr0866 |
Dihydroorotate dehydrogenase |
C575T |
P192L |
||
|
spr0878 |
Exoribonuclease R |
G280T |
G94W |
||
|
spr0895 |
Conserved hypothetical protein |
G134C |
R45T |
||
|
spr0917 |
Citrulline cluster-linked gene |
C59T |
A20V |
||
|
spr0934 |
ABC transporter substrate-binding protein - iron transport |
G486A |
W162* |
||
|
spr1041 |
Hypothetical protein |
C648T |
SYN |
||
|
spr1043 |
Conserved hypothetical protein |
C109T |
P37S |
C105T |
SYN |
|
spr1092 |
tRNA pseudouridine 5S synthase |
C210T |
SYN |
||
|
spr1127 |
Ribonuclease III |
A228G |
SYN |
C554T |
T185I |
|
spr1152 |
LicD |
G692T |
P231L |
||
|
spr1166 |
Signal recognition particle (Fifty four homolog) |
C139T |
P47S |
||
|
spr1178 |
Hypothetical protein |
C82T |
Q28* |
C82T |
Q28* |
|
spr1186 |
N-Acetylneuraminate lyase subunit, truncation |
T603C |
SYN |
||
|
G620A |
G207D |
||||
|
GAC660AAT |
T221I |
||||
|
AA689CG |
E230A |
||||
|
T696A |
SYN |
||||
|
spr1224 |
Conserved hypothetical protein |
T564C |
SYN |
||
|
spr1240 |
Alanyl-tRNA synthetase |
A139G |
T47A |
||
|
spr1254 |
ABC transporter ATP-binding protein-phosphate transport (PstB) |
G499A |
G167S |
C614T |
T205I |
|
spr1260 |
Conserved hypothetical protein |
T597C |
SYN |
||
|
spr1272 |
N-Acetylglucosamine-6-phosphate isomerase |
G385A |
G129R |
||
|
spr1384 |
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysine ligase |
G996A |
M332I |
||
|
spr1423 |
Conserved hypothetical protein |
A673G |
M225V |
||
|
spr1453 |
Major facilitator superfamily transporter |
T91C |
F31L |
||
|
spr1465 |
Conserved hypothetical protein |
G419A |
G140E |
||
|
spr1517 |
Penicillin-binding protein 2b |
C1245A |
D415E |
C1184T |
A395V |
|
C1528A |
G665D |
G1303A |
G435S |
||
|
A1351G |
T451A |
||||
|
spr1587 |
Conserved hypothetical protein |
C568T |
SYN |
||
|
spr1703 |
ABC transporter ATP-binding protein - oligopeptide transport |
C575A |
P192Q |
||
|
spr1706 |
ABC transporter membrane-spanning permease - oligopeptide transport |
T399deletion |
frameshift |
||
|
spr1862 |
Competence protein |
A51G |
SYN |
||
|
spr1886 |
Degenerate transposase |
A314G |
*105W |
||
|
spr1888 |
DNA mismatch repair protein (HexA) |
C2183T |
T728I |
C976T |
Q326* |
|
spr1991 |
Glycerol kinase |
GG78TT |
E27* |
||
|
|
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|
Asterisks and SYN indicate nonsense mutations and synonymous mutations, respectively. |
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|
Fani et al. Genome Biology 2011 12:R115 doi:10.1186/gb-2011-12-11-r115 |
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