Quantitative RT-PCR analyses of sense transcripts following siRNA-mediated knockdown of antisense transcripts. In response to heat shock, the sense transcripts are down-regulated. These transcripts contain an antisense Alu in the genic region with a predicted HSF site that may drive antisense transcription. Following siRNA-mediated knockdown of the antisense transcripts (signal in Affymetrix antisense Exon array), sense transcripts were up-regulated in response to heat shock. Error bars represent standard deviation among experimental replicates.
Pandey et al. Genome Biology 2011 12:R117 doi:10.1186/gb-2011-12-11-r117