Figure 8.

HSF occupancy within Alus in the proximal region of antisense transcripts. The possibility that putative high-score HSF sites within Alus drive antisense transcription was confirmed by ChIP-PCR using ChIP DNA made against HSF1. In response to heat shock, we observed enriched binding of HSF1 at its predicted site compared to the untreated state. The absence of a band in the 'no antibody' (No Ab) control points towards conditional and specific binding of HSF1 to the putative site.

Pandey et al. Genome Biology 2011 12:R117   doi:10.1186/gb-2011-12-11-r117
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