Figure 3.

Cumulative size distributions of smORFs with conserved start and stop codons. (a, b) Size distributions (represented as cumulative graphs) for the putative D. melanogaster smORFs with tBLASTn E-value < 1 × 10^-3 (a) or < 0.05 (b), and conserved, in-frame start and stop codons in D. pseudoobscura (SS), and their respective controls composed of reverse stop-to-start control 'smORFs' passing the same filters. The candidate 'real' smORF distributions are very different from the controls representing random short DNA sequences. For a statistical analysis of the differences between these distributions, see Additional file 1.