Figure 3.

TraSH ratios of cofactor biosynthetic gene groups. (a) Average TraSH ratios from the initial selection for those cofactor biosynthetic genes predicted by Nmb_iTM560 to be essential. (b) Average competitive indices (the change in TraSH ratios) following sequential culturing on Columbia Agar Base (CAB, clear bars), serum (lined bars) and minimal media (MM) agar (filled bars) for those cofactor biosynthetic genes predicted by Nmb_iTM560 to be essential. Values are the average of five biological replicates (three for sera). All the Nmb_iTM560 predicted essential genes for ubiquinone and riboflavin synthesis were also predicted to be essential by TraSH during the initial selection and so are missing from the minimal media agar and serum selections.