Impact on expression of transcript lengths adjustment. Smooth scatterplot of the log fold change in transcript length after adjusting for non-uniform read generation vs. the log fold change in expression. The hundred transcripts in the lowest density regions are shown as black dots. Changes in the expression estimates tend to be inversely proportional to adjustments to the lengths but for some transcripts even a small change in the length has a significant impact on the expression estimate.
Turro et al. Genome Biology 2011 12:R13 doi:10.1186/gb-2011-12-2-r13