Additional file 8.

Figure S3. RT-qPCR identification of stable endogenous genes. (a) Determining the most stable reference genes using the average expression stability value M of the remaining reference genes during a stepwise exclusion of the least stable internal control gene. The genes are ranked according to increasing expression stability. At the left are the least stable genes and at the right are the most stable ones. (b) Determining the optimal number of reference genes using the pairwise variation V between two sequential normalization factors containing an increasing number of genes with 0.15 as a proposed cutoff value by Vandesompele et al. [90].

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Vercruysse et al. Genome Biology 2011 12:R17   doi:10.1186/gb-2011-12-2-r17