Genome Biology

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Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries

Daniel Aird, Michael G Ross, Wei-Sheng Chen, Maxwell Danielsson, Timothy Fennell, Carsten Russ, David B Jaffe, Chad Nusbaum and Andreas Gnirke*

Genome Biology 2011, 12:R18 doi:10.1186/gb-2011-12-2-r18

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Current challenges in de novo plant genome sequencing and assembly

Michael C Schatz, Jan Witkowski, W Richard McCombie Genome Biology 2012, 13:243 (27 April 2012)

Michael Schatz and colleagues assess challenges in de novo plant genome assembly and review the best practices for the plant community

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Gene fragmentation in bacterial draft genomes: extent, consequences and mitigation

Jonathan L Klassen, Cameron R Currie BMC Genomics 2012, 13:14 (10 January 2012)

Methodology article   Open Access Highly Accessed

Optimizing illumina next-generation sequencing library preparation for extremely at-biased genomes

Samuel O Oyola, Thomas D Otto, Yong Gu, Gareth Maslen, Magnus Manske, Susana Campino, Daniel J Turner, Bronwyn MacInnis, Dominic P Kwiatkowski, Harold P Swerdlow, Michael A Quail BMC Genomics 2012, 13:1 (3 January 2012)

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Targeted high throughput sequencing in clinical cancer Settings: formaldehyde fixed-paraffin embedded (FFPE) tumor tissues, input amount and tumor heterogeneity

Martin Kerick, Melanie Isau, Bernd Timmermann, Holger Sültmann, Ralf Herwig, Sylvia Krobitsch, Georg Schaefer, Irmgard Verdorfer, Georg Bartsch, Helmut Klocker, Hans Lehrach, Michal R Schweiger BMC Medical Genomics 2011, 4:68 (29 September 2011)