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Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries

Daniel Aird1, Michael G Ross1, Wei-Sheng Chen2, Maxwell Danielsson2, Timothy Fennell3, Carsten Russ1, David B Jaffe1, Chad Nusbaum1 and Andreas Gnirke1*

Author Affiliations

1 Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, 320 Charles Street, Cambridge, MA 02141, USA

2 Learning Community C, Cambridge Rindge and Latin School, 459 Broadway, Cambridge, MA 02138, USA

3 Genome Sequencing Platform, Broad Institute of MIT and Harvard, 320 Charles Street, Cambridge, MA 02141, USA

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Genome Biology 2011, 12:R18  doi:10.1186/gb-2011-12-2-r18

Published: 21 February 2011

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Additional file 1:

Supplementary Figures 1 to 6.

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Additional file 2:

Supplementary Tables 1 to 3.

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