Table 1

Percentage of bases covered at less than one-tenth of the mean 'PER'-wide coverage

PCR condition

P. falciparum

E. coli

R. sphaeroides

'PER'


Phusion HF short (standard) denaturation, fast ramp

41%

0.59%

95%

42%

Phusion HF long denaturation, 2M betaine

45%

0.00011%

0.0096%

33%

AccuPrime Taq HiFi long denaturation, extension at 65°C

20%

0.00015%

0.032%

14%

AccuPrime Taq HiFi long denaturation, extension at 60°C

8.8%

0.00017%

0.085%

6.4%


Aird et al. Genome Biology 2011 12:R18   doi:10.1186/gb-2011-12-2-r18

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