Figure 9.

Positional bias correction effect on expression. This figure shows the effective length fold change due to positional bias correction for the SRA012427 dataset. So that the parameters would be consistent for all transcripts, we have limited the analysis to transcripts with length greater than 2,433, which is the largest of the 5 length bins we use for measuring positional bias. As expected, all single isoform genes are adjusted in the same way, whereas isoform deconvolution is altered based on the difference in relative position within a transcript for a read that maps to multiple isoforms.