Method
Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis
- † Equal contributors
1 School of Biological Sciences, University of Liverpool, Crown Street, Liverpool L69 7ZB, UK
2 Department of Plant Physiology, Umea Plant Science Centre, Umea University, SE-901 87 Umea, Sweden
3 Applied Biosystems, 120 Birchwood Boulevard, Warrington WA3 7QH, UK
4 Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, Mayfield Road, Edinburgh EH9 3JH, UK
Genome Biology 2011, 12:R28 doi:10.1186/gb-2011-12-3-r28
Published: 23 March 2011Abstract
Map based cloning in Arabidopsis thaliana can be a difficult and time-consuming process, specifically if the phenotype is subtle and scoring labour intensive. Here, we have re-sequenced the 120-Mb genome of a novel Arabidopsis clock mutant early bird (ebi-1) in Wassilewskija (Ws-2). We demonstrate the utility of sequencing a backcrossed line in limiting the number of SNPs considered. We identify a SNP in the gene AtNFXL-2 as the likely cause of the ebi-1 phenotype.
