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Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis

Kevin Ashelford1, Maria E Eriksson2, Christopher M Allen3, Rosalinda D'Amore1, Mikael Johansson2, Peter Gould1, Suzanne Kay1, Andrew J Millar4, Neil Hall1* and Anthony Hall1*

Author Affiliations

1 School of Biological Sciences, University of Liverpool, Crown Street, Liverpool L69 7ZB, UK

2 Department of Plant Physiology, Umea Plant Science Centre, Umea University, SE-901 87 Umea, Sweden

3 Applied Biosystems, 120 Birchwood Boulevard, Warrington WA3 7QH, UK

4 Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, Mayfield Road, Edinburgh EH9 3JH, UK

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Genome Biology 2011, 12:R28  doi:10.1186/gb-2011-12-3-r28

Published: 23 March 2011

Abstract

Map based cloning in Arabidopsis thaliana can be a difficult and time-consuming process, specifically if the phenotype is subtle and scoring labour intensive. Here, we have re-sequenced the 120-Mb genome of a novel Arabidopsis clock mutant early bird (ebi-1) in Wassilewskija (Ws-2). We demonstrate the utility of sequencing a backcrossed line in limiting the number of SNPs considered. We identify a SNP in the gene AtNFXL-2 as the likely cause of the ebi-1 phenotype.