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Open Access Method

ISsaga is an ensemble of web-based methods for high throughput identification and semi-automatic annotation of insertion sequences in prokaryotic genomes

Alessandro M Varani*, Patricia Siguier, Edith Gourbeyre, Vincent Charneau and Mick Chandler*

Author Affiliations

Laboratoire de Microbiologie et Génétique Moléculaires, CNRS 118, Route de Narbonne, 31062 Toulouse Cedex, France

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Genome Biology 2011, 12:R30  doi:10.1186/gb-2011-12-3-r30

Published: 28 March 2011

Abstract

Insertion sequences (ISs) play a key role in prokaryotic genome evolution but are seldom well annotated. We describe a web application pipeline, ISsaga (http://issaga.biotoul.fr/ISsaga/issaga_index.php webcite), that provides computational tools and methods for high-quality IS annotation. It uses established ISfinder annotation standards and permits rapid processing of single or multiple prokaryote genomes. ISsaga provides general prediction and annotation tools, information on genome context of individual ISs and a graphical overview of IS distribution around the genome of interest.