Figure 2.

Base calling robustness is affected by probe length and melting temperature. Mean D_score is plotted by (a) probe length and (b) probe melting temperature where vertical lines indicate 95% confidence intervals. Lower discrimination scores indicate greater base calling robustness. Fixed length 39-mers provided the best performance for any static probe length tested. Probes with a 66°C melting temperature provided the best performance for any melting temperature range and surpassed the performance of 39-mer probes.