Figure 2.

Base calling robustness is affected by probe length and melting temperature. Mean Dscore is plotted by (a) probe length and (b) probe melting temperature where vertical lines indicate 95% confidence intervals. Lower discrimination scores indicate greater base calling robustness. Fixed length 39-mers provided the best performance for any static probe length tested. Probes with a 66°C melting temperature provided the best performance for any melting temperature range and surpassed the performance of 39-mer probes.

Tan et al. Genome Biology 2011 12:R35   doi:10.1186/gb-2011-12-4-r35
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